Sophie Buysse

Hi there! I'm Sophie (she/her), a fourth year PhD Candidate at Michigan State University.

I'm co-advised by Emily Josephs and Jeff Conner in the Plant Biology Department and the Ecology, Evolution, and Behavior Program. I am broadly interested in plant evolution. The main themes in my dissertation work are adaptation to changing climates and the interaction of genetic drift and selection in trait loss.

Research

I research plant evolution by using the model organism Arabidopsis thaliana. I'm particularly interested in adaptation to changing climates. My dissertation research includes two main projects.

selfie of Sophie in front of a growth chamber filled with flats and pots

The role of plasticity in adaptation to climate change

I am fascinated by the ability of plants to respond to their environment and to adapt to stressful environments. In this project, I am investigating the role of phenotypic plasticity in adpatation to climate change by incorporating within and between population variation in plasticity, selection gradients for plasticity, and quantitative trait loci (QTL) analysis to start to uncover the genetic architecture of plasticity for multiple traits. This project is composed of chamber common gardens where I grow native genotypes and recombinant inbred lines of A. thaliana in simulated current and future environments while measuring a suite of traits related to phenology, drought response, and fitness. I aim to answer three main questions:
1. Is there population differentiation for plasticity?
2. Do more plastic genotypes have higher fitness in the future environment? And are these genotypes native lines or recombinant inbred lines?
3. Are QTL underlying drought response traits and fitness similar in the current and future environments? Can we identify QTL for plasticity?
The results from this project will be applicable in understanding the effect of assisted migration and improving crops for climate resiliency.

Check out the work I've presented thus far on this project!

Evolution 2022 Poster - pilot study results
Evolution 2023 Poster - first results from RILs

image of short stamens; credit to Frances Whalen

Constraint of Trait Loss

Traits are maintained through a balance of genetic drift and selection. When traits have no impact on fitness, we generally expect them to be lost through drift or selection against the trait. In this project, I am investigating the roles of genetic drift and selection in short stamen loss in A. thaliana. Previous evidence shows that short stamens do not contribute to fitness in A. thaliana, but they have been retained. I am investigating why using populations along an elevation gradient in the Spanish Pyrenees. I also use genome wide association studies to look for loci associated with short stamen number.

You can find my preprint here.

Teaching

Scientific literacy is a critical skill for navigating our world. Whether through media or informal conversations, we are confronted with scenarios that require scientific literacy skills such as identifying valid arguments, interpreting data, and justifying conclusions. These skills are transferable outside the classroom and are appropriate goals for teaching introductory biology classes where students have diverse backgrounds and diverse career goals. My own teaching-as-research project and the work of others has shown that we struggle to teach students science literacy skills (Gormally et al. 2012). Thus, my teaching aims to center science literacy through (1) cooperative learning, (2) inquiry, and (3) reflection focused on keeping content relevant to real world applications and scenarios where students may need these skills in their everyday life or their future career.

As preparation for my role as an educator, I am working to complete a Certificate in College Teaching through the Graduate School at MSU. My portfolio will be updated as I progress through the program:

Certificate In College Teaching Portfolio

image of Sophie in front of a scientific poster with a model of the project

Teaching as research

As a Future Academic Scholar in Teaching at MSU, I designed and implemented a teaching-as-research project that used a modelling activity in an introductory biology lab to emphasize to students that science and society are connected. A common goal of introductory biology labs is to increase student science literacy. My project focused on the important question: how do models influence how students understsand the connections between science and society? Students took science literacy surveys at the beginning and end of the semester, and half of the students completed a modelling activity during the semester. Ultimately, I found that there was no increase in science literacy for students, whether they completed the modelling activity or not. These results indicate the important role of assessing science literacy and adjusting our instruction to make sure we accomplish our teaching goals.

You can find out more about this project under Competency 5 in my Certificate In College Teaching.

image of Tori in front of a scientific poster

Mentoring

My approach to mentoring is that each student has individualized needs as a mentee; my role is to teach mentees the skills they need to design effective experiments while increasing confidence in their problem solving and critical thinking skills.

Mentored students with independent projects include: Evan Adamski (MSU Undergrad, Spring 2024), Tori Nicholes (KBS REU student, 2022 - pictured with her poster), Trevor Markwood (MSU Undergrad, 2022-2023), Athena Dila (Plant Genomics REU student, 2023). I also mentor two students who assist with my research projects but do not yet have independent projects and interact with other Josephs Lab undergraduates.

CV

Check out my CV here
In short, my resarch skills include:

  • Bioinformatics and statistical analysis
  • Growing and phenotyping plants
  • Literature Review
  • Molecular techniques
  • Outreach and Mentoring
  • Drinking Tea

Contact Me

The best way to contact me is to send me an email